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Characterization of Hantaan Virions, the Prototype Virus of Hemorrhagic Fever with Renal Syndrome

Connie S. Schmaljohn, Sherman E. Hasty, Stephen A. Harrison and Joel M. Dalrymple
The Journal of Infectious Diseases
Vol. 148, No. 6 (Dec., 1983), pp. 1005-1012
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30133826
Page Count: 8
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Characterization of Hantaan Virions, the Prototype Virus of Hemorrhagic Fever with Renal Syndrome
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Abstract

Hantaan virus, strain 76-118, was propagated to high titer in a clone of Vero cells, and infectious virions were successfully concentrated and purified. Infectivity and virus antigenic activity were closely associated with a virus particle that exhibited a sedimentation rate indistinguishable from a representative member of the Bunyaviridae. Purified virions sedimented to a density of 1.16-1.17 in sucrose and 1.20-1.21 in cesium chloride. Detergent disruption of virions resulted in a nucleocapsid structure (density, 1.18 in sucrose and 1.25 in cesium chloride) and soluble protein antigens. Three separate nucleocapsids were resolved by rate-zonal centrifugation and contained a single but common polypeptide of 50,000 daltons. Electrophoresis of radiolabeled RNA extracted from purified virions yielded a profile of three RNA species with apparent molecular weights of 2.7, 1.2, and $0.6 \times 10^{6}$. These data support earlier electron microscopy reports which suggested that Hantaan virus has characteristics similar to some members of the virus family Bunyaviridae.

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