You are not currently logged in.
Access JSTOR through your library or other institution:
Regulation of Expression of Herpes Simplex Virus (HSV) Glycoprotein D in Vaccinia Recombinants Affects Their Ability to Protect from Cutaneous HSV-2 Disease
M. Wachsman, L. Aurelian, C. C. Smith, M. E. Perkus and E. Paoletti
The Journal of Infectious Diseases
Vol. 159, No. 4 (Apr., 1989), pp. 625-634
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30137089
Page Count: 10
You can always find the topics here!Topics: Human herpesvirus 2, Viruses, Antigens, Infections, T lymphocytes, Vaccinia virus, Viral diseases, Vaccinia, Skin diseases, Simplexvirus
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Preview not available
The effect of regulation of herpes simplex virus (HSV) type 1 glycoprotein D (gD-1) gene expression on HSV-specific immune response and protection from cutaneous HSV-2 disease was studied using vaccinia virus recombinants containing gD-1 under the control of early (VP176) or late (VP254) vaccinia virus promoters. Expression of gD-1 in VP176- infected cells was first observed at 2 h after infection. It did not depend on viral DNA replication. In VP254-infected cells, gD-1 was first observed at 24 h after infection and its expression depended on DNA replication. Immunized guinea pigs had similar titers of HSV-specific neutralizing antibody. However, HSV-specific T cell responses were significantly higher in VP176- than in VP254-immunized animals as determined by lymphoproliferation (P < .005) and delayed type hypersensitivity (P < .01). The reduced T cell responses of VP254-immunized guinea pigs correlated with poor gD-1 expression in VP254-infected antigen presenting cells (splenic adherent and epidermal cells). Immunization with VP176, but not with VP254, protected guinea pigs from primary (P < .0005) and recurrent (P < .0005) cutaneous HSV-2 lesions.
The Journal of Infectious Diseases © 1989 Oxford University Press