Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

The Molecular Epidemiology of Three Biotypes of Corynebacterium diphtheriae in the Seattle Outbreak, 1972-1982

Marie B. Coyle, Neal B. Groman, Jennifer Q. Russell, James P. Harnisch, Myron Rabin and King K. Holmes
The Journal of Infectious Diseases
Vol. 159, No. 4 (Apr., 1989), pp. 670-679
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/30137095
Page Count: 10
  • Download ($42.00)
  • Subscribe ($19.50)
  • Cite this Item
The Molecular Epidemiology of Three Biotypes of Corynebacterium diphtheriae in the Seattle Outbreak, 1972-1982
Preview not available

Abstract

Between 1972 and 1982 Seattle experienced a diphtheria outbreak involving 1,100 cases, primarily adults with cutaneous lesions. Biotyping revealed three consecutive overlapping outbreaks including 433 toxinogenic intermedius cases. Isolates from each quarter year were examined for DNA restriction fragment patterns and hybridization patterns with three DNA probes. All intermedius outbreak isolates appeared identical in all analyses and most stock cultures had the outbreak DNA fragment pattern, but DNA probes detected six patterns within 11 intermedius stock cultures. The mitis outbreak was heterogeneous, involving at least five restriction fragment patterns. In contrast, DNA restriction fragment analyses indicated that 22 of 25 gravis outbreak isolates belonged to a single strain, whereas there were seven strains within eight gravis stock cultures. DNA probe analyses of gravis outbreak isolates detected seven different patterns, five involving copy numbers of the toxB fragment and attB sites that presumably reflected lysogenic events that occurred during the outbreak.

Page Thumbnails

  • Thumbnail: Page 
670
    670
  • Thumbnail: Page 
671
    671
  • Thumbnail: Page 
672
    672
  • Thumbnail: Page 
673
    673
  • Thumbnail: Page 
674
    674
  • Thumbnail: Page 
675
    675
  • Thumbnail: Page 
676
    676
  • Thumbnail: Page 
677
    677
  • Thumbnail: Page 
678
    678
  • Thumbnail: Page 
679
    679