Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Adrenergic Effects on the Oxygen Affinity and pH of Cultured Erythrocytes and Blood of the Mummichog, Fundulus heteroclitus

Paula M. Dalessio, Leonard DiMichele and Dennis A. Powers
Physiological Zoology
Vol. 64, No. 6 (Nov. - Dec., 1991), pp. 1407-1425
Stable URL: http://www.jstor.org/stable/30158222
Page Count: 19
  • Read Online (Free)
  • Download ($19.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Adrenergic Effects on the Oxygen Affinity and pH of Cultured Erythrocytes and Blood of the Mummichog, Fundulus heteroclitus
Preview not available

Abstract

Epinephrine had no effect on the blood O₂ afinity of Fundulus heteroclitus in the presence of low CO₂ (1.5 mmHg Pco₂). However, it did increase O₂ afinity proportionately with increasing Pco₂. Erythrocytes in NaHCO₃-buffered Bull saline gave results that were virtually identical to those obtained with blood. This was not the case for erythrocytes in synthetically buffered Bull salines. Their O₂ afinity was markedly lower than that of blood, regardless of the CO₂ concentration, presence of epinephrine or bovine serum albumin, or type of synthetic buffer (Hepes, Tris, Bis-Tris) employed. Thus, conclusions drawn from studies utilizing synthetically buffered salines may be misleading Epinephrine stimulated proton effux, resulting in an increase of the intracellular pH from erythrocytes in Hepes-buffered Bull saline. This appears to be an artifact resulting from the use of Hepes, as it was not observed in blood. The intracellular pH of both blood and erythrocytes in NaHCO₃-buffered Bull saline was insensitive to beta-adrenergic stimulation. This finding is contrary to those reported for trout and striped bass and may reflect adaptive responses of F. heteroclitus to its often poorly oxygenated estuarine habitat.

Page Thumbnails

  • Thumbnail: Page 
1407
    1407
  • Thumbnail: Page 
1408
    1408
  • Thumbnail: Page 
1409
    1409
  • Thumbnail: Page 
1410
    1410
  • Thumbnail: Page 
1411
    1411
  • Thumbnail: Page 
1412
    1412
  • Thumbnail: Page 
1413
    1413
  • Thumbnail: Page 
1414
    1414
  • Thumbnail: Page 
1415
    1415
  • Thumbnail: Page 
1416
    1416
  • Thumbnail: Page 
1417
    1417
  • Thumbnail: Page 
1418
    1418
  • Thumbnail: Page 
1419
    1419
  • Thumbnail: Page 
1420
    1420
  • Thumbnail: Page 
1421
    1421
  • Thumbnail: Page 
1422
    1422
  • Thumbnail: Page 
1423
    1423
  • Thumbnail: Page 
1424
    1424
  • Thumbnail: Page 
1425
    1425