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Induction of High Titers of Anti-IgE by Immunization of Inbred Mice with Syngeneic IgE
Seiji Haba and Alfred Nisonoff
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 14 (Jul. 15, 1987), pp. 5009-5013
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/30319
Page Count: 5
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We have generated high titers (up to 1 mg/ml) of antibodies to isotypic determinants of IgE by immunization of A/J mice with syngeneic monoclonal IgE conjugated to keyhole limpet hemocyanin. As much as 3 mg of antiidiotypic antibodies per ml was induced at the same time. In contrast to conventional rheumatoid factors, the anti-isotypic antibodies are of moderately high affinity (107-108 M-1). Assays of the anti-IgE antisera indicated the presence of IgE, both free and in the form of immune complexes; the latter values are minimum estimates owing to masking of isotypic determinants. Regulatory effects of these high titers of anti-IgE can now be investigated. Such studies will be facilitated by the availability of monoclonal, syngeneic anti-IgE antibodies.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences