Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

A Yeast-like mRNA Capping Apparatus in Plasmodium falciparum

C. Kiong Ho and Stewart Shuman
Proceedings of the National Academy of Sciences of the United States of America
Vol. 98, No. 6 (Mar. 13, 2001), pp. 3050-3055
Stable URL: http://www.jstor.org/stable/3055187
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Yeast-like mRNA Capping Apparatus in Plasmodium falciparum
Preview not available

Abstract

Analysis of the mRNA capping apparatus of the malaria parasite Plasmodium falciparum illuminates an evolutionary connection to fungi rather than metazoans. We show that P. falciparum encodes separate RNA guanylyltransferase (Pgt1) and RNA triphosphatase (Prt1) enzymes and that the triphosphatase component is a member of the fungal/viral family of metal-dependent phosphohydrolases, which are structurally and mechanistically unrelated to the cysteine-phosphatase-type RNA triphosphatases found in metazoans and plants. These results highlight the potential for discovery of mechanism-based antimalarial drugs designed to specifically block the capping of Plasmodium mRNAs. A simple heuristic scheme of eukaryotic phylogeny is suggested based on the structure and physical linkage of the triphosphatase and guanylyltransferase enzymes that catalyze cap formation.

Page Thumbnails

  • Thumbnail: Page 
3050
    3050
  • Thumbnail: Page 
3051
    3051
  • Thumbnail: Page 
3052
    3052
  • Thumbnail: Page 
3053
    3053
  • Thumbnail: Page 
3054
    3054
  • Thumbnail: Page 
3055
    3055