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The Role of Distinct p185neu Extracellular Subdomains for Dimerization with the Epidermal Growth Factor (EGF) Receptor and EGF-Mediated Signaling
Toru Kumagai, James G. Davis, Takeo Horie, Donald M. O'Rourke and Mark I. Greene
Proceedings of the National Academy of Sciences of the United States of America
Vol. 98, No. 10 (May 8, 2001), pp. 5526-5531
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3055660
Page Count: 6
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The extracellular domain of p185c-neu can be viewed as a complex structure of four subdomains, two of which are cysteine-rich subdomains. We have investigated the contribution of these distinct p185c-neu extracellular subdomains to p185/epidermal growth factor receptor (EGFR) heteromer formation and EGF-induced heteromeric signaling. Our studies indicate that at least two separate p185 subdomains, a region spanning subdomains I and II and subdomain IV are involved in association of p185 with the EGFR. We also demonstrated that subdomain IV reduced the heteromeric signaling and transforming activities induced by EGF after associating with EGFR. When 126 aa were deleted from subdomain IV, this small subdomain IV-derived fragment could still lead to heterodimers with EGFR and suppress EGF-induced mitogen-activated protein kinase activation and subsequent transformation abilities. These data provide information about trans-inhibitory mechanisms of mutant p185 species and also indicate that both the entire and a part of subdomain IV may represent a therapeutic target for erbB-overexpressing tumors. Finally, these studies define a basic feature of receptor-receptor associations that are determined by cystine-knot containing subdomains.
Proceedings of the National Academy of Sciences of the United States of America © 2001 National Academy of Sciences