Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Growth Inhibition and DNA Damage Induced by Cre Recombinase in Mammalian Cells

Ate Loonstra, Marc Vooijs, H. Berna Beverloo, Bushra Al Allak, Ellen van Drunen, Roland Kanaar, Anton Berns and Jos Jonkers
Proceedings of the National Academy of Sciences of the United States of America
Vol. 98, No. 16 (Jul. 31, 2001), pp. 9209-9214
Stable URL: http://www.jstor.org/stable/3056325
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Growth Inhibition and DNA Damage Induced by Cre Recombinase in Mammalian Cells
Preview not available

Abstract

The use of Cre/loxP recombination in mammalian cells has expanded rapidly. We describe here that Cre expression in cultured mammalian cells may result in a markedly reduced proliferation and that this effect is dependent on the endonuclease activity of Cre. Chromosome analysis after Cre expression revealed numerous chromosomal aberrations and an increased number of sister chromatid exchanges. Titration experiments in mouse embryo fibroblasts with a ligand-regulatable Cre-ERT show that toxicity is dependent on the level of Cre activity. Prolonged, low levels of Cre activity permit recombination without concomitant toxicity. This urges for a careful titration of Cre activity in conditional gene modification in mammalian cells.

Page Thumbnails

  • Thumbnail: Page 
9209
    9209
  • Thumbnail: Page 
9210
    9210
  • Thumbnail: Page 
9211
    9211
  • Thumbnail: Page 
9212
    9212
  • Thumbnail: Page 
9213
    9213
  • Thumbnail: Page 
9214
    9214