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Growth Inhibition and DNA Damage Induced by Cre Recombinase in Mammalian Cells
Ate Loonstra, Marc Vooijs, H. Berna Beverloo, Bushra Al Allak, Ellen van Drunen, Roland Kanaar, Anton Berns and Jos Jonkers
Proceedings of the National Academy of Sciences of the United States of America
Vol. 98, No. 16 (Jul. 31, 2001), pp. 9209-9214
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3056325
Page Count: 6
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The use of Cre/loxP recombination in mammalian cells has expanded rapidly. We describe here that Cre expression in cultured mammalian cells may result in a markedly reduced proliferation and that this effect is dependent on the endonuclease activity of Cre. Chromosome analysis after Cre expression revealed numerous chromosomal aberrations and an increased number of sister chromatid exchanges. Titration experiments in mouse embryo fibroblasts with a ligand-regulatable Cre-ERT show that toxicity is dependent on the level of Cre activity. Prolonged, low levels of Cre activity permit recombination without concomitant toxicity. This urges for a careful titration of Cre activity in conditional gene modification in mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America © 2001 National Academy of Sciences