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Accurate 5′ Splice-Site Selection in Mouse κ Immunoglobulin Light Chain Premessenger RNAs is not Cell-Type-Specific
Dean H. Kedes and Joan A. Steitz
Proceedings of the National Academy of Sciences of the United States of America
Vol. 84, No. 22 (Nov. 15, 1987), pp. 7928-7932
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/30574
Page Count: 5
You can always find the topics here!Topics: Splicing, HeLa cells, RNA, Exons, Introns, Lymphocytes, Transfection, Untranslated regions, Genes, Cell lines
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In mature mouse B lymphocytes, immunoglobulin κ light chain transcripts contain an intervening sequence separating the recombined variable (V) plus joining (J) exon from the distant constant (C) exon. After V--J recombination, this intervening sequence can include as many as three unused but very similar J-region 5′ splice sites. Each of these sites is potentially functional if the gene is appropriately recombined. It is unclear how the splicing machinery distinguishes among these 5′ splice sites, always choosing the most upstream site. We used synthetic transcripts of κ gene sequences containing J3 and J4 in both the germ-line and the recombined configurations to study the pattern of 5′ splice-site selection in vitro. We find that both HeLa cell and lymphocyte nuclear extracts fail to discriminate between the J3- and J4-region 5′ splice sites. In contrast, after transfection into HeLa cells, similar κ light chain transcripts are spliced correctly at the most upstream 5′ splice site--that which is used in κ -producing cells. We conclude that accurate 5′ splice-site selection in the mouse κ light chain is neither cell-type- nor species-specific. Potential mechanisms for this controlling step in gene expression are discussed.
Proceedings of the National Academy of Sciences of the United States of America © 1987 National Academy of Sciences