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Genome Sequence of the Hyperthermophilic Crenarchaeon Pyrobaculum aerophilum
Sorel T. Fitz-Gibbon, Heidi Ladner, Ung-Jin Kim, Karl O. Stetter, Melvin I. Simon and Jeffrey H. Miller
Proceedings of the National Academy of Sciences of the United States of America
Vol. 99, No. 2 (Jan. 22, 2002), pp. 984-989
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3057677
Page Count: 6
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We determined and annotated the complete 2.2-megabase genome sequence of Pyrobaculum aerophilum, a facultatively aerobic nitrate-reducing hyperthermophilic Topt = 100°C) crenarchaeon. Clues were found suggesting explanations of the organism's surprising intolerance to sulfur, which may aid in the development of methods for genetic studies of the organism. Many interesting features worthy of further genetic studies were revealed. Whole genome computational analysis confirmed experiments showing that P. aerophilum (and perhaps all crenarchaea) lack 5′ untranslated regions in their mRNAs and thus appear not to use a ribosome-binding site (Shine-Dalgarno)-based mechanism for translation initiation at the 5′ end of transcripts. Inspection of the lengths and distribution of mononucleotide repeat-tracts revealed some interesting features. For instance, it was seen that mononucleotide repeat-tracts of Gs (or Cs) are highly unstable, a pattern expected for an organism deficient in mismatch repair. This result, together with an independent study on mutation rates, suggests a "mutator" phenotype.
Proceedings of the National Academy of Sciences of the United States of America © 2002 National Academy of Sciences