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Chronic Intracellular Infection of Alfalfa Nodules by Sinorhizobium meliloti Requires Correct Lipopolysaccharide Core
Gordon R. O. Campbell, Bradley L. Reuhs and Graham C. Walker
Proceedings of the National Academy of Sciences of the United States of America
Vol. 99, No. 6 (Mar. 19, 2002), pp. 3938-3943
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3058230
Page Count: 6
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Our analyses of lipopolysaccharide mutants of Sinorhizobium meliloti offer insights into how this bacterium establishes the chronic intracellular infection of plant cells that is necessary for its nitrogen-fixing symbiosis with alfalfa. Derivatives of S. meliloti strain Rm1021 carrying an lpsB mutation are capable of colonizing curled root hairs and forming infection threads in alfalfa in a manner similar to a wild-type strain. However, developmental abnormalities occur in the bacterium and the plant at the stage when the bacteria invade the plant nodule cells. Loss-of-function lpsB mutations, which eliminate a protein of the glycosyltransferase I family, cause striking changes in the carbohydrate core of the lipopolysaccharide, including the absence of uronic acids and a 40-fold relative increase in xylose. We also found that lpsB mutants were sensitive to the cationic peptides melittin, polymyxin B, and poly-L-lysine, in a manner that paralleled that of Brucella abortus lipopolysaccharide mutants. Sensitivity to components of the plant's innate immune system may be part of the reason that this mutant is unable to properly sustain a chronic infection within the cells of its host-plant alfalfa.
Proceedings of the National Academy of Sciences of the United States of America © 2002 National Academy of Sciences