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Essential Role for Cholesterol in Entry of Mycobacteria into Macrophages

John Gatfield and Jean Pieters
Science
New Series, Vol. 288, No. 5471 (Jun. 2, 2000), pp. 1647-1650
Stable URL: http://www.jstor.org/stable/3075484
Page Count: 4
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Abstract

Mycobacteria are intracellular pathogens that can invade and survive within host macrophages, thereby creating a major health problem worldwide. The molecular mechanisms involved in mycobacterial entry are still poorly characterized. Here we report that cholesterol is essential for uptake of mycobacteria by macrophages. Cholesterol accumulated at the site of mycobacterial entry, and depleting plasma membrane cholesterol specifically inhibited mycobacterial uptake. Cholesterol also mediated the phagosomal association of TACO, a coat protein that prevents degradation of mycobacteria in lysosomes. Thus, by entering host cells at cholesterol-rich domains of the plasma membrane, mycobacteria may ensure their subsequent intracellular survival in TACO-coated phagosomes.

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