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Induction of Expression of Cell-Surface Homologous Restriction Factor upon Anti-CD3 Stimulation of Human Peripheral Lymphocytes
Dale E. Martin, Leora S. Zalman and Hans J. Müller-Eberhard
Proceedings of the National Academy of Sciences of the United States of America
Vol. 85, No. 1 (Jan. 1, 1988), pp. 213-217
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/31039
Page Count: 5
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Homologous restriction factor (HRF) is a 65-kDa membrane protein that inhibits transmembrane channel formation by the membrane-attack complex of complement and by the complement component C9-related cytolytic lymphocyte protein. Stimulation of resting peripheral human lymphocytes with the anti-CD3 monoclonal antibody OKT3 has been shown to induce cytotoxicity in the CD8+ subpopulation. As demonstrated here, OKT3 stimulation also induces expression of cell-surface HRF by CD4+ and CD8+ T lymphocytes. The small proportion of Leu 19+ natural killer lymphocytes present in peripheral blood mononuclear cells was found to express HRF prior to stimulation. Whereas unstimulated peripheral blood mononuclear cells were susceptible to lysis by the membrane-attack complex or by the C9-related protein, OKT3-stimulated peripheral blood mononuclear cells were relatively resistant to both the membrane-attack complex and C9-related protein. This acquired resistance was abrogated by blocking surface HRF with F(ab′)2 anti-HRF, suggesting that resistance was due to lymphocyte-membrane HRF. By using solid-phase anti-HRF, a 65-kDa protein was isolated from the activated peripheral blood mononuclear cells and shown to be capable of conferring upon sheep erythrocytes the characteristic activity of human HRF.
Proceedings of the National Academy of Sciences of the United States of America © 1988 National Academy of Sciences