You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The C-Terminal Domain Phosphatase and Transcription Elongation Activities of FCP1 are Regulated by Phosphorylation
Erika M. Friedl, William S. Lane, Hediye Erdjument-Bromage, Paul Tempst and Danny Reinberg
Proceedings of the National Academy of Sciences of the United States of America
Vol. 100, No. 5 (Mar. 4, 2003), pp. 2328-2333
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3139527
Page Count: 6
You can always find the topics here!Topics: Phosphorylation, Phosphatases, Gels, HeLa cells, Elution, Proteins, Monoclonal antibodies, Ion traps, Phosphoproteins, Immunoprecipitation
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII) is heavily phosphorylated during the transition from transcription initiation to the establishment of an elongation-competent transcription complex. FCP1 is the only phosphatase known to be specific for the CTD of the largest subunit of RNAPII, and its activity is believed to be required to reactivate RNAPII, so that RNAPII can enter another round of transcription. We demonstrate that FCP1 is a phosphoprotein, and that phosphorylation regulates FCP1 activities. FCP1 is phosphorylated at multiple sites in vivo. The CTD phosphatase activity of phosphorylated FCP1 is stimulated by TFIIF, whereas dephosphorylated FCP1 is not. In addition to its role in the recycling of RNAPII, FCP1 also affects transcription elongation. Phosphorylated FCP1 is more active in stimulating transcription elongation than the dephosphorylated form of FCP1. We found that only phosphorylated FCP1 can physically interact with TFIIF. We set out to purify an FCP1 kinase from HeLa cells and identified casein kinase 2, which, surprisingly, displayed a negative effect on FCP1-associated activities.
Proceedings of the National Academy of Sciences of the United States of America © 2003 National Academy of Sciences