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T Cell Chemotaxis to Lysophosphatidylcholine through the G2A Receptor
Caius G. Radu, Li V. Yang, Mireille Riedinger, Matthew Au and Owen N. Witte
Proceedings of the National Academy of Sciences of the United States of America
Vol. 101, No. 1 (Jan. 6, 2004), pp. 245-250
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3148405
Page Count: 6
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G2A is an immunoregulatory G protein-coupled receptor predominantly expressed in lymphocytes and macrophages. Ectopic overexpression studies have implicated G2A as a receptor for the bioactive lysophospholipid, lysophosphatidylcholine (LPC). However, the functional consequences of LPC-G2A interaction at physiological levels of receptor expression, and in a cellular context relevant to its immunological role, remain largely unknown. Here, we show impaired chemotaxis to LPC of a T lymphoid cell line in which G2A expression was chronically down-regulated by RNA interference technology. Rescuing this phenotype by reconstitution of the physiological level of receptor expression further supports a functional connection between LPC-G2A interaction and cellular motility. Overexpression of G2A in the T lymphoid cell line significantly enhanced chemotaxis to LPC. It also modified migration toward the LPC-related molecule, lysophosphatidic acid, indicating the possibility of crosstalk between G2A and endogenous lysophosphatidic acid receptors. The role of G2A in LPC-mediated cell migration may be relevant to the autoimmune syndrome associated with genetic inactivation of this G protein-coupled receptor in mice. The experimental system described here can be useful for understanding the structural requirements for LPC recognition by G2A and the signaling pathways regulated by this ligand-receptor pair.
Proceedings of the National Academy of Sciences of the United States of America © 2004 National Academy of Sciences