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Histochemistry of Mucosubstances in the Mantle of the Clam, Mercenaria mercenaria. II. MucoSubstances in the Second Marginal Fold

Robert E. Hillman
Transactions of the American Microscopical Society
Vol. 88, No. 3 (Jul., 1969), pp. 420-425
Published by: Wiley on behalf of American Microscopical Society
DOI: 10.2307/3224710
Stable URL: http://www.jstor.org/stable/3224710
Page Count: 6
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Histochemistry of Mucosubstances in the Mantle of the Clam, Mercenaria mercenaria. II. MucoSubstances in the Second Marginal Fold
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Abstract

The results of a variety of histochemical reactions on mucosubstances in neutral formalin-fixed tissues indicate that in the second fold of the mantle edge there are two mucous cell types, one secreting a sulfated acid mucopolysaccharide or glycosaminoglycan and the other forming a neutral mucosubstance. The neutral mucosubstance was PAS-positive prior to and following diastase digestion. It also exhibited a positive PAS reaction following staining with alcian blue at pH 2.5, both prior to and following hyaluronidase digestion. For the most part, the glycosaminoglycan stained intensely with alcian blue at pH 2.5 and somewhat less intensely at pH 1.0. Portions of the material retained alcianophilia in solutions of magnesium chloride up to 1.0 molar, and after testicular hyaluronidase digestion. The material also reacted strongly with iron diamine and aldehyde fuchsin when these reagents were followed by alcian blue. Methylation extinguished alcianophilia in the acid mucosubstance, but it was partially restored following saponification.

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