You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Histochemistry of Mucosubstances in the Mantle of the Clam, Mercenaria mercenaria. I. A Glycosaminoglycan in the First Marginal Fold
Robert E. Hillman
Transactions of the American Microscopical Society
Vol. 87, No. 3 (Jul., 1968), pp. 361-367
Stable URL: http://www.jstor.org/stable/3224822
Page Count: 7
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Neutral formalin-fixed sections of the mantle from the quahog clam Mercenaria mercenaria were subjected to a variety of histochemical reactions for mucosubstances. The results of these reactions on the first, or outer, fold indicate that a sulfated connective tissue glycosaminoglycan is secreted beneath the outer epithelium in the area where most of the new shell is deposited. The mucus is strongly alcianophilic at pH 1.0 and 2.5, and the alcianophilia persists when the tissues are stained with alcian blue in concentrations of up to 0.6 m magnesium chloride. The material also reacts strongly with aldehyde fuchsin and high iron diamine when these reagents are followed by alcian blue. Interestingly, the mucus is also periodic acid-Schiff positive, which is unusual for sulfated connective tissue mucosubstances. Testicular hyaluronidase has no effect on the staining characteristics of the mucus. Because of the localization of the mucus in the shell-forming area of the mantle, and positive von Kossa reactions for calcium, it is suggested that this material may play some role in shell deposition.
Transactions of the American Microscopical Society © 1968 American Microscopical Society