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The Use of Fluorescent Protein End-Group Methods in the Study of Slug Oocyte Nucleoli

Ronald R. Cowden
Transactions of the American Microscopical Society
Vol. 93, No. 3 (Jul., 1974), pp. 374-378
Published by: Wiley on behalf of American Microscopical Society
DOI: 10.2307/3225437
Stable URL: http://www.jstor.org/stable/3225437
Page Count: 5
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The Use of Fluorescent Protein End-Group Methods in the Study of Slug Oocyte Nucleoli
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Abstract

Five fluorescent cytochemical end-group methods were used to reexamine oogenesis in the slug Limax flavus, particularly the unusual multiple nucleoli in these cells. The method for demonstrating protein SH groups failed entirely to fluorochrome nucleoli. In contrast, nucleoli were intensely fluorescent when the same fluorochrome was used after thioglycollate reduction. Since the most intense fluorescence was localized in the "nucleolar cap" of the "amphinucleolus" complex, SS groups are probably involved in the assembly of ribosomes. While larger primary nucleoli displayed more intense fluorescence than smaller secondary nucleoli after methods directed toward the demonstration of C-terminal and side-chain carboxyl groups, the methods for demonstrating SS groups and basic groups resulted in equivalent levels of fluorescence in both types of nucleoli. These results were discussed in relation to the special value of fluorescence methods for the study of nucleoli and oogenesis in general.

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