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A Simple Chromosome Spread Technique for Unarmored Dinoflagellates and Implications of Polyploidy in Algal Cultures
C. L. Loper, K. A. Steidinger and L. M. Walker
Transactions of the American Microscopical Society
Vol. 99, No. 3 (Jul., 1980), pp. 343-346
Stable URL: http://www.jstor.org/stable/3226012
Page Count: 4
You can always find the topics here!Topics: Chromosomes, Diploidy, Polyploidy, Haploidy, Cultured cells, Cell culture techniques, Ploidies, Plant tissues, Aneuploidy, Somatic cells
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Chromosome counts can be used to verify stages of dinoflagellate life cycles. However, standard chromosome techniques do not work well with dinoflagellate nuclei. A simple method involving cell lysis, heat, desiccation, and staining has been effective for Ptychodiscus brevis (Davis) Steidinger. Two vegetative count ranges were determined with this technique: (1) a haploid number of 121 ± 3 for field and recent culture specimens; and (2) a diploid number of 240 ± 6 for a 25-year old culture. The latter probably is a result of autodiploidy during extended culture conditions. Polyploidy may be occurring in many long-established algal cultures; therefore, the genetic status of present culture collections should be determined and stocks perhaps replaced.
Transactions of the American Microscopical Society © 1980 American Microscopical Society