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Procedures for Accurate Documentation of Shapes and Dimensions of Larval Bivalve Shells with Scanning Electron Microscopy
S. Cynthia Fuller, Richard A. Lutz and Alan Pooley
Transactions of the American Microscopical Society
Vol. 108, No. 1 (Jan., 1989), pp. 58-63
Stable URL: http://www.jstor.org/stable/3226207
Page Count: 6
You can always find the topics here!Topics: Magnification, Larvae, Larval development, Calibration, Photomicrographs, Electrons, Microscopes, Electron micrographs, Databases, Diameters
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Shapes of larval bivalve shells documented with a scanning electron microscope can be grossly distorted because of minor misadjustments of machine settings. In particular, orthogonal accuracy is necessary; i.e., the magnification must be equal and accurate in both the horizontal and vertical directions. Calculated magnifications from the display system of the microscope are only approximate, vary with time and with maintenance visits, and are not adequate for discrimination of specimens within a similar size range. Preliminary calibration steps and the use of a standard grid minimize potential sources of error resulting from limitations of the microscope.
Transactions of the American Microscopical Society © 1989 American Microscopical Society