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Computerized Image Analysis of Resin-Embedded Hair

W. M. Hess and R. E. Seegmiller
Transactions of the American Microscopical Society
Vol. 107, No. 4 (Oct., 1988), pp. 421-425
Published by: Wiley on behalf of American Microscopical Society
DOI: 10.2307/3226338
Stable URL: http://www.jstor.org/stable/3226338
Page Count: 5
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Computerized Image Analysis of Resin-Embedded Hair
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Abstract

Perimeter and area of cross-sectioned hair was studied with computer analysis to quantitate morphological differences between head hair and whiskers. Samples were treated in basic and acid solutions to condition them before embedding, sectioning, and staining. Stained slides were projected and areas were traced. For each sample, 50 randomly selected individual cross-sections of hairs were traced and numbered. Inked drawings were scanned by an image analyzer connected to an Apple II microcomputer equipped with a digitizer board, and the t-test was used to determine differences between average perimeter and areas for head hair and whiskers from the same Caucasian individual. By use of these procedures, we were able to determine that the average perimeter and area for cross-sectioned whiskers were 2.60 and 4.26 times greater, respectively, than head hair.

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