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Ultrastructural Localization of ATPases in the Gastrodermis of Megalodiscus temperatus with Additional Observations on Schistosoma japonicum (Trematoda: Digenea)

Burton J. Bogitsh
Transactions of the American Microscopical Society
Vol. 109, No. 3 (Jul., 1990), pp. 311-318
Published by: Wiley on behalf of American Microscopical Society
DOI: 10.2307/3226801
Stable URL: http://www.jstor.org/stable/3226801
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Ultrastructural Localization of ATPases in the Gastrodermis of Megalodiscus temperatus with Additional Observations on Schistosoma japonicum (Trematoda: Digenea)
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Abstract

The strontium-lead protocol was used to localize, ultrastructurally, a ouabain-sensitive, potassium-dependent p-nitrophenyl phosphatase (K-pNPPase) of the Na,KATPase complex in the gastrodermis of the frog rectal fluke Megalodiscus temperatus and the blood fluke Schistosoma japonicum. K-pNPPase activity was observed on the cytoplasmic side of the gastrodermal microvillus plasma membrane of Megalodiscus and extracellularly on the parenchymal side of the gastrodermal basal plasma membrane and its infoldings of Schistosoma. A ouabain-insensitive ATPase, possibly Mg-ATPase, and nonspecific acid phosphatase activities were observed extracellularly on the luminal sides of the gastrodermal amplifications of both organisms. Active transport of Na+ via Na,KATPase probably provides the driving force for the transport of solutes such as glucose, amino acids, etc. from the lumen into the gastrodermis of M. temperatus. This mechanism is either absent or much reduced in schistosomes, and the mediated transport of such molecules as glucose and amino acids is the function of their teguments. The ouabain-sensitive ATPase in the basal plasma membrane of the schistosome gastrodermis may function in ionic regulation. The function of the tegument in the acquisition of nutrients is believed to determine the type of transport in the digestive tract.

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