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Molecular Cloning of an Inducible Serine Esterase Gene from Human Cytotoxic Lymphocytes
Joseph A. Trapani, Jared L. Klein, Perrin C. White and Bo Dupont
Proceedings of the National Academy of Sciences of the United States of America
Vol. 85, No. 18 (Sep. 15, 1988), pp. 6924-6928
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/32494
Page Count: 5
You can always find the topics here!Topics: T lymphocytes, Natural killer cells, Cell lines, RNA, Complementary DNA, Amino acids, Lymphocytes, Mice, Messenger RNA, DNA
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A cDNA clone encoding a human serine esterase gene was isolated from a library constructed from poly(A)+ RNA of allogeneically stimulated, interleukin 2-expanded peripheral blood mononuclear cells. The clone, designated HSE26.1, represents a full-length copy of a 0.9-kilobase mRNA present in human cytotoxic cells but absent from a wide variety of noncytotoxic cell lines. Clone HSE26.1 contains an 892-base-pair sequence, including a single 741-base-pair open reading frame encoding a putative 247-residue polypeptide. The first 20 amino acids of the polypeptide form a leader sequence. The mature protein is predicted to have an unglycosylated Mr of ≈ 26,000 and contains a single potential site for N-linked glycosylation. The nucleotide and predicted amino acid sequences of clone HSE26.1 are homologous with all murine and human serine esterases cloned thus far but are most similar to mouse granzyme B (70% nucleotide and 68% amino acid identity). HSE26.1 protein is expressed weakly in unstimulated peripheral blood mononuclear cells but is strongly induced within 6-hr incubation in medium containing phytohemagglutinin. The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity.
Proceedings of the National Academy of Sciences of the United States of America © 1988 National Academy of Sciences