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Observations on Hearts Explanted In vitro from the Snail Australorbis glabratus

Eli Chernin
The Journal of Parasitology
Vol. 49, No. 3 (Jun., 1963), pp. 353-364
DOI: 10.2307/3275797
Stable URL: http://www.jstor.org/stable/3275797
Page Count: 12
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Observations on Hearts Explanted In vitro from the Snail Australorbis glabratus
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Abstract

Hemolymph analyses, technical procedures, and culture solutions are described which made possible the study of whole, trypsin-treated hearts of Australorbis glabratus explanted in plastic culture flasks. Cells of two types, amebocytic and "epithelial," emerged from explants and persisted in apparently good condition for varying lengths of time. Cell division was not observed. The explanted hearts underwent certain gross and histologic alterations, but the organs retained their essential integrity in vitro, and heartbeat was observed for at least 47 days after explantation.

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