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A Simplified Method for the In vitro Cultivation of the Rat Tapeworm, Hymenolepis diminuta

Everett L. Schiller
The Journal of Parasitology
Vol. 51, No. 4 (Aug., 1965), pp. 516-518
DOI: 10.2307/3276218
Stable URL: http://www.jstor.org/stable/3276218
Page Count: 3
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Simplified Method for the In vitro Cultivation of the Rat Tapeworm, Hymenolepis diminuta
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Abstract

Hymenolepis diminuta has been grown in vitro from the cysticercoid to the patent adult stage using a diffused diphasic medium consisting of a blood-agar base (NNN) overlaid with Hanks' balanced salt solution. The culture flasks were incubated at 37 C in a Dubnoff metabolic shaking incubator under a gas mixture of 97% N2-3% CO2. Details of methodology and data on the growth and development of worms cultivated in this simplified system are reported.

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