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Improved Method for the Monoxenic Cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like Amebae with Trypanosomatids

Louis S. Diamond
The Journal of Parasitology
Vol. 54, No. 4 (Aug., 1968), pp. 715-719
DOI: 10.2307/3277027
Stable URL: http://www.jstor.org/stable/3277027
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Improved Method for the Monoxenic Cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like Amebae with Trypanosomatids
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Abstract

An improved method is described for the monoxenic cultivation of Entamoeba histolytica and E. histolytica-like amebae in association with trypanosomatids. Trophozoites taken directly from ameba-bacteria cultures and treated with antibiotics, or cysts microisolated from similar cultures are introduced along with the associates, either Crithidia sp. or Trypanosoma cruzi, into a specially devised liquid medium consisting of Tryptose, Trypticase, yeast extract, glucose, cysteine, ascorbic acid, salts, serum, and hemolyzed blood. The same medium suffices for maintenance of the stock cultures of the crithidia, while a simplified version is used for the trypanosome stocks. Technically, the new method offers certain advantages over those described earlier: The new media are easier to prepare, and the procedures for growing and subculturing the amebae are simpler, principally because preconditioned medium is not employed as it is in the older techniques. The fact that the Entamoeba-Crithidia cultures provide the investigator with the only ameba-trypanosomatid system in which the associate is not infectious for vertebrates is especially significant for those contemplating in vivo studies, and those hesitant about the handling of a pathogen such as T. cruzi.

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