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Differentiation of Entamoeba: Encystation of E. invadens in Monoxenic and Axenic Cultures
Varunee Thepsuparungsikul, Lui Seng and Gordon B. Bailey
The Journal of Parasitology
Vol. 57, No. 6 (Dec., 1971), pp. 1288-1292
Stable URL: http://www.jstor.org/stable/3277981
Page Count: 5
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A method to stimulate mass encystation of Entamoeba invadens in monoxenic or axenic cultures, using a simple monophasic medium, has been developed. The procedure involves transfer of trophozoites from an axenic growth medium (TPS-1) to an encystation medium containing serum which has been conditioned by growth of bacteria, either prior (axenic) or subsequent (monoxenic) to inoculation with amebae. In encystation media lacking conditioned serum trophozoites do not encyst and generally lyse. Several strains of bacteria can effectively condition the encystation medium; best results were obtained using Proteus morganii. Axenically produced cysts are indistinguishable from monoxenic cysts and excysted in growth medium.
The Journal of Parasitology © 1971 The American Society of Parasitologists