Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Differentiation of Entamoeba: Encystation of E. invadens in Monoxenic and Axenic Cultures

Varunee Thepsuparungsikul, Lui Seng and Gordon B. Bailey
The Journal of Parasitology
Vol. 57, No. 6 (Dec., 1971), pp. 1288-1292
DOI: 10.2307/3277981
Stable URL: http://www.jstor.org/stable/3277981
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Differentiation of Entamoeba: Encystation of E. invadens in Monoxenic and Axenic Cultures
Preview not available

Abstract

A method to stimulate mass encystation of Entamoeba invadens in monoxenic or axenic cultures, using a simple monophasic medium, has been developed. The procedure involves transfer of trophozoites from an axenic growth medium (TPS-1) to an encystation medium containing serum which has been conditioned by growth of bacteria, either prior (axenic) or subsequent (monoxenic) to inoculation with amebae. In encystation media lacking conditioned serum trophozoites do not encyst and generally lyse. Several strains of bacteria can effectively condition the encystation medium; best results were obtained using Proteus morganii. Axenically produced cysts are indistinguishable from monoxenic cysts and excysted in growth medium.

Page Thumbnails

  • Thumbnail: Page 
1288
    1288
  • Thumbnail: Page 
1289
    1289
  • Thumbnail: Page 
1290
    1290
  • Thumbnail: Page 
1291
    1291
  • Thumbnail: Page 
1292
    1292