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Synchronization of Plasmodium falciparum Erythrocytic Stages in Culture

Chris Lambros and Jerome P. Vanderberg
The Journal of Parasitology
Vol. 65, No. 3 (Jun., 1979), pp. 418-420
DOI: 10.2307/3280287
Stable URL: http://www.jstor.org/stable/3280287
Page Count: 3
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Synchronization of Plasmodium falciparum Erythrocytic Stages in Culture
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Abstract

Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single and multiple ring-form infections. At the same time, varying degrees of lysis of erythrocytes infected with the more mature stages of the parasite was evident. Approximately 95% of the parasites were in the ring stage of development at 48 and 96 hr after sorbitol treatment; likewise, a high percentage of trophozoite and schizont stages was observed at 24, 72, and 120 hr. D-Mannitol produced similar, selective, lytic effects.

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