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Identification of the Structural Component in the Cyst Wall of Entamoeba invadens

Angel Arroyo-Begovich, Alfonso Cárabez-Trejo and José Ruíz-Herrera
The Journal of Parasitology
Vol. 66, No. 5 (Oct., 1980), pp. 735-741
DOI: 10.2307/3280662
Stable URL: http://www.jstor.org/stable/3280662
Page Count: 7
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Identification of the Structural Component in the Cyst Wall of Entamoeba invadens
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Abstract

Cyst walls of Entamoeba invadens were isolated and purified. Both whole cysts and purified walls appeared intensely fluorescent when stained with Calcofluor white M2R. Examination of positive replicas of purified cyst walls with the electron microscope revealed the presence of a microfibrillar structure. The main sugars detected in acid hydrolysates from the walls were hexosamines. X-ray diffraction analysis of purified cyst walls demonstrated that the crystalline polymer present was chitin.

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