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Culture of Plasmodium falciparum: The Role of pH, Glucose, and Lactate
Mona D. Jensen, Margaret Conley and Lucy Dale Helstowski
The Journal of Parasitology
Vol. 69, No. 6 (Dec., 1983), pp. 1060-1067
Stable URL: http://www.jstor.org/stable/3280864
Page Count: 8
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Yields of P. falciparum in intraerythrocytic in vitro cultures were maximized when extracellular pH was maintained between 7.2 and 7.45, and extracellular lactate was kept below 12 mM. Host erythrocytes metabolized
$4.6\;\pm\;1.5\;\muM$ glucose/109 RBC/24 hr and produced $7.9\;\pm\;1.8\muM$ lactate/109 RBC/24 hr. Asynchronous parasite cultures used $122\;\pm\;34\;\muM$ glucose/109 parasitized RBC/24 hr and produced $143\;\pm\;47\muM$ lactate/109 parasitized RBC/24 hr. Synchronous cultures that were 80 to 100% ring forms after 24 hr in culture exhibited significantly lower glycolysis per 109 parasitized RBC than cultures that were 0 to 25% ring forms after 24 hr. The percent of glucose utilization accounted for by lactate production by parasites was significantly less than that of uninfected erythrocytes. These optimum ranges and metabolic rates can be used in the development of parasite culture techniques.
The Journal of Parasitology © 1983 The American Society of Parasitologists