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Effect of Cytochalasin-B, Low Ca++ Concentration, Iodoacetic Acid, and Quinacrine-HCl on the Attachment of Giardia Trophozoites In vitro
Dennis E. Feely and Stanley L. Erlandsen
The Journal of Parasitology
Vol. 68, No. 5 (Oct., 1982), pp. 869-873
Stable URL: http://www.jstor.org/stable/3280996
Page Count: 5
You can always find the topics here!Topics: Trophozoites, Giardiasis, Microvilli, Actins, Rats, Cell motility, Contractile proteins, HeLa cells, Viability, Microtubules
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Giardia sp. trophozoites were isolated directly from the small intestines of rats and permitted to attach to polystyrene Petri dishes incubated at 37 C. Attached trophozoites were treated in vitro with various agents which inhibit cell motility (cytochalasin-B, low Ca++, colchicine) and metabolism (iodoacetic acid, 2,4-dinitrophenol) and chemotherapeutic agents (quinacrine-HCl, metronidazole). Trophozoite attachment was sensitive to cytochalasin-B, low Ca++, iodoacetate, and quinacrine-HCl. Contractile proteins (actin, myosin) have been demonstrated previously in the periphery of the ventral disc. The effect of cytochalasin-B and low Ca++ concentrations on trophozoite attachment is consistent with the suggested role of contractile proteins in the mechanism of attachment. The effect of iodoacetate suggests that energy for attachment is derived from glycolysis. The effect of quinacrine-HCl, on attachment was rapid (< 10 min with 10.0 μg/ml). Its mode of action on attachment is not understood.
The Journal of Parasitology © 1982 The American Society of Parasitologists