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The Influence of Cell Type and Culture Medium on the In vitro Cultivation of Exoerythrocytic Stages of Plasmodium berghei

Michael R. Hollingdale, Pamela Leland, James L. Leef and Richard L. Beaudoin
The Journal of Parasitology
Vol. 69, No. 2 (Apr., 1983), pp. 346-352
DOI: 10.2307/3281232
Stable URL: http://www.jstor.org/stable/3281232
Page Count: 7
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The Influence of Cell Type and Culture Medium on the In vitro Cultivation of Exoerythrocytic Stages of Plasmodium berghei
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Abstract

Plasmodium berghei sporozoites successfully entered and developed into exoerythrocytic schizonts in a variety of cell types cultured in vitro, but segmentation and release of merozoites was only observed in human embryonic lung cells. Exoerythrocytic development was generally not influenced by the culture medium, and NCTC-135 was used routinely. In vitro infectivity of P. berghei sporozoites was unaffected by the serum type used for isolation.

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