You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Bacterial Endosymbionts of Acanthamoeba sp.
John Hall and Herbert Voelz
The Journal of Parasitology
Vol. 71, No. 1 (Feb., 1985), pp. 89-95
Stable URL: http://www.jstor.org/stable/3281982
Page Count: 7
You can always find the topics here!Topics: Endosymbionts, Trophozoites, Cysts, Cytoplasm, Bacteria, Electrons, Capsules, Parasitology, Cell membranes, Broths
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Gimenez staining of presumably axenic Acanthamoeba sp., strain HN-3, showed rod-shaped cytoplasmic inclusions. Electron microscopy of thin sections of the amebae showed these to be bacilli which measured 1.3 to 3.3 μm by 0.22 to 0.33 μm. Their cell envelopes were those typical of gram-negative bacteria, surrounded by an electron-translucent area that stains with ruthenium red, suggesting the presence of a capsule. The bacilli grew and reproduced in the cytoplasm of both trophozoites and cysts of Acanthamoeba sp. There was no evidence of a surrounding phagosomal or phagolysosomal membrane. They were retained by the ameba both during encystment and excystment. All attempts to isolate the endosymbionts in embryonated eggs and/or standard bacteriological media failed; and they persisted within the amebae for 1 to 6 mo despite temperature shocking or constant treatment of cultures with penicillin, streptomycin, chloramphenicol, tetracycline, erythromycin, polymyxin B, ampicillin, isoniazid, rifampicin, or gentamycin at concentrations of 10-5 to 10-3 M.
The Journal of Parasitology © 1985 The American Society of Parasitologists