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Field Inversion Gel Electrophoretic Separation of Cryptosporidium spp. Chromosome-Sized DNA
Jan R. Mead, Michael J. Arrowood, William L. Current and Charles R. Sterling
The Journal of Parasitology
Vol. 74, No. 3 (Jun., 1988), pp. 366-369
Stable URL: http://www.jstor.org/stable/3282039
Page Count: 4
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Chromosomal DNA from 5 isolates of Cryptosporidium parvum and 1 of C. baileyi were compared by field-inversion gel electrophoresis (FIGE). FIGE analyses of parasite DNA prepared from purified sporozoites versus intact oocysts showed no observable differences. Chromosomal DNA migration patterns of the 5 C. parvum isolates were indistinguishable, whereas similar but distinct differences were evident between C. baileyi and the isolates of C. parvum. Oocyst-reactive monoclonal antibodies differentiated oocysts of C. parvum from those of C. baileyi but were unable to distinguish oocysts of 1 isolate of C. parvum from another.
The Journal of Parasitology © 1988 The American Society of Parasitologists