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Isolation of Cryptosporidium Oocysts and Sporozoites Using Discontinuous Sucrose and Isopycnic Percoll Gradients
Michael J. Arrowood and Charles R. Sterling
The Journal of Parasitology
Vol. 73, No. 2 (Apr., 1987), pp. 314-319
Stable URL: http://www.jstor.org/stable/3282084
Page Count: 6
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Techniques for the large-scale isolation of Cryptosporidium oocysts and sporozoites, obtained from the feces of experimentally infected Holstein calves, were developed employing discontinuous sucrose gradients and isopycnic Percoll gradients. The oocyst recovery method utilized 2 sequential discontinuous sucrose gradients followed by 1 Percoll gradient. Recovered oocysts were essentially free of debris and bacteria and represented 34% of the original oocyst suspension. Sporozoites were recovered from excystation mixtures on a single Percoll gradient. Sixty-three percent of the original sporozoites were recovered with 2.2% contamination by intact oocysts and virtually no oocyst walls.
The Journal of Parasitology © 1987 The American Society of Parasitologists