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A Dot-ELISA Mimicry Western Blot Test for the Detection of Swine Trichinellosis
Xinzhuan Su and Annie K. Prestwood
The Journal of Parasitology
Vol. 77, No. 1 (Feb., 1991), pp. 76-82
Stable URL: http://www.jstor.org/stable/3282561
Page Count: 7
You can always find the topics here!Topics: Antigens, Enzyme linked immunosorbent assay, Infections, Antibodies, Larvae, Parasitology, Veterinary medicine, Trichinosis, False positive errors, Epitopes
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A dot enzyme-linked immunosorbent assay (dot-ELISA) using antigens purified by monoclonal antibody affinity chromatography was developed for detecting Trichinella spiralis infection in swine. The test was as sensitive as an ELISA using excretory-secretory products as antigen and western blot analysis, and nearly as specific as the western blot. The dot-ELISA detected all of 20 low infections (0.08-4.74 larvae per gram of diaphragm), most of them by 5-6 wk postinfection. Sera from 1,960 farm-reared swine were tested by conventional ELISA, dot-ELISA, and western blot. Of the 1,960 sera, 262 (13.4%) were considered positive on conventional ELISA, 16 (0.82%) by dot-ELISA, and 15 (0.77%) by western blot. The improved specificity was achieved by employing species-specific denatured antigens. More importantly, the dot-ELISA was much simpler to perform than western blot analysis. The principles employed in this test can be adapted to other infectious diseases, such as AIDS.
The Journal of Parasitology © 1991 The American Society of Parasitologists