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Axenic Isolation of Viable Giardia muris Trophozoites
K. D. Tillotson, A. Buret and M. E. Olson
The Journal of Parasitology
Vol. 77, No. 3 (Jun., 1991), pp. 505-508
Stable URL: http://www.jstor.org/stable/3283149
Page Count: 4
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Large numbers of viable Giardia muris trophozoites were isolated from the duodenum of experimentally infected mice 6 days after inoculation with 1,000 G. muris cysts. A series of shaking, incubation, and washing steps in the presence of the broad-spectrum antibiotic piperacillin readily provided 4.9 ± 1.5 × 105 G. muris trophozoites per mouse, free of detectable contaminant organisms. Anaerobic and microaerophilic culturing and scanning electron microscopy demonstrated axenic status and high purity of the isolates. The viability of trophozoites was 98 ± 2%. Application of this technique should permit novel immunological and epidemiological analyses of G. muris infection and biochemical investigations of this protozoan parasite.
The Journal of Parasitology © 1991 The American Society of Parasitologists