Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Characterization of Eimeria tenella Unsporulated Oocyst-Specific cDNA Clones

R. G. Herbert, J. J. Pasternak and M. A. Fernando
The Journal of Parasitology
Vol. 78, No. 6 (Dec., 1992), pp. 1011-1018
DOI: 10.2307/3283222
Stable URL: http://www.jstor.org/stable/3283222
Page Count: 8
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Characterization of Eimeria tenella Unsporulated Oocyst-Specific cDNA Clones
Preview not available

Abstract

A cDNA library was constructed with poly(A)+ RNA from unsporulated oocysts of Eimeria tenella in pUC18. After screening, 4 cDNA clones that hybridized to RNA of unsporulated and sporulating oocysts but not to RNA of either sporulated oocysts or second generation merozoites were isolated and characterized. Each of the cDNA clones is unique. The loci for 2 of the clones are on E. tenella chromosome 7, the site of the third is located on chromosome 6 and the last clone hybridizes, for the most part, to chromosome 5 but also to other E. tenella chromosomes. The cognate RNAs for each of the cDNA clones show differential patterns of hybridization during oocyst sporulation with the levels of RNA being low at the start of sporulation (0 hr), increasing to peak levels between 6.5 and 23 hr after the onset of sporulation and, in each case, decreasing to low hybridization levels at 48 hr after initiation of sporulation. These results establish that specific mRNA levels are differentially regulated during sporulation.

Page Thumbnails

  • Thumbnail: Page 
1011
    1011
  • Thumbnail: Page 
1012
    1012
  • Thumbnail: Page 
1013
    1013
  • Thumbnail: Page 
1014
    1014
  • Thumbnail: Page 
1015
    1015
  • Thumbnail: Page 
1016
    1016
  • Thumbnail: Page 
1017
    1017
  • Thumbnail: Page 
1018
    1018