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Collection of Eggs and Hatching and Culturing Second-Stage Larvae of Toxocara vitulorum In vitro
R. P. V. J. Rajapakse, V. W. S. M. Vasanthathilake, S. Lloyd and S. T. Fernando
The Journal of Parasitology
Vol. 78, No. 6 (Dec., 1992), pp. 1090-1092
Stable URL: http://www.jstor.org/stable/3283237
Page Count: 3
You can always find the topics here!Topics: Larvae, Eggs, Hatching, Parasitology, Antigens, Chemical suspensions, Larval development, Ova, Visceral larva migrans, Animal collection
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Eggs of Toxocara vitulorum were harvested from the feces of infected buffalo calves and embryonated in vitro. Optimum conditions for hatch and culture of the second-stage larvae were determined. Maximum hatch of larvae occurred from eggs that were decoated by treatment with saturated Ca(OCl)2 for 16-24 min followed by treatment with CO2 and incubation at 37 C. Larvae could be cultured in RPMI-1640 medium for up to 3 mo but survived for only 3 wk in Eagle's minimum essential medium.
The Journal of Parasitology © 1992 The American Society of Parasitologists