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Cultivation of Leishmania braziliensis in an Economical Serum-Free Medium Containing Human Urine

Timothy C. Armstrong and Jean L. Patterson
The Journal of Parasitology
Vol. 80, No. 6 (Dec., 1994), pp. 1030-1032
DOI: 10.2307/3283454
Stable URL: http://www.jstor.org/stable/3283454
Page Count: 3
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Cultivation of Leishmania braziliensis in an Economical Serum-Free Medium Containing Human Urine
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Abstract

Leishmania braziliensis cells are difficult to culture in vitro and usually require media supplemented with serum for sustained cell division. Fresh, sterile urine is an inexpensive substitute for serum in the culture of 2 strains of L. braziliensis, 1 infected with Leishmania RNA virus 1, and 1 uninfected. In the presence of urine, both the infected and the uninfected strains grew to the same final cell density as the same strains grown in the presence of serum. One strain of Leishmania major was also successfully cultured in urine-supplemented media.

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