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Use of RAPD-PCR to Differentiate Genetically Defined Lines of an Intermediate Host of Schistosoma mansoni, Biomphalaria glabrata
Sally E. Larson, Peter L. Andersen, Andre N. Miller, Carolyn E. Cousin, Charles S. Richards, Fred A. Lewis and Matty Knight
The Journal of Parasitology
Vol. 82, No. 2 (Apr., 1996), pp. 237-244
Stable URL: http://www.jstor.org/stable/3284154
Page Count: 8
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The genetic differentiation among several laboratory-maintained pedigree snail lines of Biomphalaria glabrata (with different susceptibility phenotypes to Schistosoma mansoni infection) was assessed with the random amplified polymorphic DNA method. Out of the 20 primers tested, 2 (OPA-01 and OPA-06) gave reproducible markers with either individual or bulked DNA samples from resistant (BS-90, 10-R2, LAC-line) and susceptible (M-line) snails. Arbitrary primer, OPA-01, amplification of BS-90 DNA identified a 180-bp strain-specific fragment and a 400-bp marker in the susceptible M-line stock. In the 10-R2 and LAC snail lines, OPA-01 specific markers of 200 bp and 550 bp were identified. Amplification with primer OPA-06 identified several major strain-specific markers in the BS-90 (150 bp, 400 bp, 800 bp) and M-line (1,100 bp) snails. The heritability of the RAPD markers was evaluated in progeny snails derived from a cross between the BS-90 and M-line stocks. Results showed that markers were inherited in a dominant or codominant fashion. The 1,100-bp M-line marker was inherited in all susceptible progeny snails analyzed.
The Journal of Parasitology © 1996 The American Society of Parasitologists