Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Mast Cell Hyperplasia and Increased Macromolecular Uptake in an Animal Model of Giardiasis

James A. Hardin, Andre G. Buret, Merle E. Olson, Michael H. Kimm and D. Grant Gall
The Journal of Parasitology
Vol. 83, No. 5 (Oct., 1997), pp. 908-912
DOI: 10.2307/3284287
Stable URL: http://www.jstor.org/stable/3284287
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Mast Cell Hyperplasia and Increased Macromolecular Uptake in an Animal Model of Giardiasis
Preview not available

Abstract

Giardiasis has been associated with an increase in allergic disease following infection suggesting an alteration in mucosal immune function. Jejunal in vivo and in vitro macromolecular transport, epithelial permeability, and mucosal and connective tissue mast cell counts were examined in Mongolian gerbils (35-45 g) orogastrically inoculated (I) with a pathogenic strain of Giardia lamblia and compared to age- and weight-matched, sham-treated controls (C) 6 and 21 days postinoculation. Macromolecular uptake was significantly increased in infected tissue at 6 days both in vivo (I 134 ± 19 vs. C 74 ± 17 ng/hr; n = 8; P < 0.05) and in vitro (I 125 ± 17 vs. C 67 ± 8 ng/hr/cm; n = 12; P < 0.05). Macromolecular uptake did not differ between groups at 21 days. Infection had no effect on mucosal permeability of [51Cr]EDTA. Mucosal mast cell counts did not differ at 6 days but were significantly elevated in infected tissue at 21 days (I 33.3 ± 6.8 vs. C 2.7 ± 0.4 per high magnification field; n = 5; P < 0.01) as were connective tissue mast cell counts (I 1.7 ± 0.2 vs. C 1.0 ± 0.1 per high magnification field; n = 13; P < 0.005). The findings indicate that during the peak phase of giardiasis, jejunal active antigen uptake is increased leading to a delayed recruitment of mucosal and connective tissue mast cells. These changes may play a role in the increased incidence of hypersensitivity reactions associated with Giardia infection.

Page Thumbnails

  • Thumbnail: Page 
908
    908
  • Thumbnail: Page 
909
    909
  • Thumbnail: Page 
910
    910
  • Thumbnail: Page 
911
    911
  • Thumbnail: Page 
912
    912