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Maintenance of Acanthamoeba culbertsoni by Cryopreservation

Ricardo Alejandre-Aguilar, Ma. Lourdes Calvo-Méndez, Benjamín Nogueda-Torres and Fernando de la Jara-Alcocer
The Journal of Parasitology
Vol. 84, No. 6 (Dec., 1998), pp. 1261-1263
DOI: 10.2307/3284685
Stable URL: http://www.jstor.org/stable/3284685
Page Count: 3
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Maintenance of Acanthamoeba culbertsoni by Cryopreservation
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Abstract

In order to determine the viability of trophozoites of Acanthamoeba culbertsoni under cryopreservation conditions, cultures in serum-casein-glucose-yeast extract medium were subject to 5%, 7.5%, and 10% concentrations of dimethylsulfoxide (DMSO). With the methodology followed, the percentages of recovery varied between 75.6% and 86.6% with DMSO at 10%, between 54.5% and 73.5% with DMSO at 7.5%, and between 43.6% and 68.5% with DMSO at 5%. The amebae were kept in liquid nitrogen for 30-210 days. The highest viability of trophozoites was founded when DMSO was used at a final concentration of 10% and an equilibrium temperature of 4 C. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.

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