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High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci

C. R. Blackston, J. P. Dubey, E. Dotson, C. Su, P. Thulliez, D. Sibley and T. Lehmann
The Journal of Parasitology
Vol. 87, No. 6 (Dec., 2001), pp. 1472-1475
DOI: 10.2307/3285322
Stable URL: http://www.jstor.org/stable/3285322
Page Count: 4
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High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci
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Abstract

High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, restriction fragment length polymorphisms, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.

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