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Resolution of Six Chromosomes of Trichomonas vaginalis and Conservation of Size and Number among Isolates

Michael W. Lehker and J. F. Alderete
The Journal of Parasitology
Vol. 85, No. 5 (Oct., 1999), pp. 976-979
DOI: 10.2307/3285842
Stable URL: http://www.jstor.org/stable/3285842
Page Count: 4
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Resolution of Six Chromosomes of Trichomonas vaginalis and Conservation of Size and Number among Isolates
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Abstract

The electrophoretic karyotype of Trichomonas vaginalis isolates was determined by contour-clamped homogeneous electric field electrophoresis. Six chromosomal bands ranging between 50 kbp and 6 Mbp were reliably resolved by our separation method. Trichomonad chromosomes fell into 3 distinct size classes. The 3 maxichromosomes were approximately 5,700, 4,700, and 3,500 kbp. Two intermediate-sized chromosomes were ~1,200 kbp and 1,100 kbp. A minichromosome was ~75 kbp. The same size and number of chromosomes were present in 15 T. vaginalis isolates obtained from different geographic regions, reinforcing the idea of a highly conserved karyotype among trichomonal isolates worldwide.

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