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Human Interleukin 7: Molecular Cloning and Growth Factor Activity on Human and Murine B-Lineage Cells
Raymond G. Goodwin, Stephen Lupton, Ann Schmierer, Kathryn J. Hjerrild, Rita Jerzy, William Clevenger, Steve Gillis, David Cosman and Anthony E. Namen
Proceedings of the National Academy of Sciences of the United States of America
Vol. 86, No. 1 (Jan. 1, 1989), pp. 302-306
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/33106
Page Count: 5
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A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.
Proceedings of the National Academy of Sciences of the United States of America © 1989 National Academy of Sciences