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Human Anti-Endoplasmic Reticulum Antibodies in Sera of Patients with Halothane-Induced Hepatitis are Directed against a Trifluoroacetylated Carboxylesterase
H. Satoh, B. M. Martin, A. H. Schulick, D. D. Christ, J. G. Kenna and L. R. Pohl
Proceedings of the National Academy of Sciences of the United States of America
Vol. 86, No. 1 (Jan. 1, 1989), pp. 322-326
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/33110
Page Count: 5
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Previous studies have demonstrated that patients with halothane-induced hepatitis have serum antibodies that are directed against novel liver microsomal neoantigens and have suggested that these neoantigens may play an immunopathological role in development of the patients' liver damage. These investigations have further revealed that the antibodies are directed against distinct polypeptide fractions (100 kDa, 76 kDa, 59 kDa, 57 kDa, 54 kDa) that have been covalently modified by the reactive trifluoroacetyl halide metabolite of halothane. In this paper, the trifluoroacetylated (TFA) 59-kDa neoantigen (59-kDa-TFA) recognized by the patients' antibodies was isolated from liver microsomes of halothane-treated rats by chromatography on an immunoaffinity column of anti-TFA IgG. Antibodies were raised against the 59-kDa-TFA protein and were used to purify the native protein from liver microsomes of untreated rats. Based upon its apparent monomeric molecular mass, NH2-terminal amino acid sequence, catalytic activity, and other physical properties, the protein has been identified as a previously characterized microsomal carboxylesterase (EC 126.96.36.199). A similar strategy may be used to purify and characterize neoantigens associated with other drug toxicities that are believed to have an immunopathological basis.
Proceedings of the National Academy of Sciences of the United States of America © 1989 National Academy of Sciences