Access

You are not currently logged in.

Access JSTOR through your library or other institution:

login

Log in through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Muscarinic Agonists and Phorbol Esters Increase Tyrosine Phosphorylation of a 40-Kilodalton Protein in Hippocampal Slices

Kathleen R. Stratton, Paul F. Worley, Richard L. Huganir and Jay M. Baraban
Proceedings of the National Academy of Sciences of the United States of America
Vol. 86, No. 7 (Apr. 1, 1989), pp. 2498-2501
Stable URL: http://www.jstor.org/stable/33506
Page Count: 4
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Muscarinic Agonists and Phorbol Esters Increase Tyrosine Phosphorylation of a 40-Kilodalton Protein in Hippocampal Slices
Preview not available

Abstract

We have used the hippocampal slice preparation to investigate the regulation of protein tyrosine phosphorylation in brain. After pharmacological treatment of intact slices, proteins were separated by electrophoresis, and levels of protein tyrosine phosphorylation were assessed by immunoblotting with specific anti-phosphotyrosine antibodies. Phorbol esters, activators of the serine- and threonine-phosphorylating enzyme protein kinase C, selectively increase tyrosine phosphorylation of a soluble protein with an apparent molecular mass of approximately 40 kilodaltons. Muscarinic agonists such as carbachol and oxotremorine M that strongly activate the inositol phospholipid system also increase tyrosine phosphorylation of this protein. Neurotransmitter activation of the inositol phospholipid system and protein kinase C appears to trigger a cascade leading to increased tyrosine phosphorylation.

Page Thumbnails

  • Thumbnail: Page 
2498
    2498
  • Thumbnail: Page 
2499
    2499
  • Thumbnail: Page 
2500
    2500
  • Thumbnail: Page 
2501
    2501