You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Control of Late Off-Center Cone Bipolar Cell Differentiation and Visual Signaling by the Homeobox Gene Vsx1
Robert L. Chow, Bela Volgyi, Rachel K. Szilard, David Ng, Colin McKerlie, Stewart A. Bloomfield, David G. Birch, Roderick R. McInnes and Jeremy Nathans
Proceedings of the National Academy of Sciences of the United States of America
Vol. 101, No. 6 (Feb. 10, 2004), pp. 1754-1759
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3371262
Page Count: 6
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Retinal bipolar cells are interneurons that transmit visual signals from photoreceptors to ganglion cells. Although the visual pathways mediated by bipolar cells have been well characterized, the genes that regulate their development and function are largely unknown. To determine the role in bipolar cell development of the homeobox gene Vsx1, whose retinal expression is restricted to a major subset of differentiating and mature cone bipolar (CB) cells, we targeted the gene in mice. Bipolar cell fate was not altered in the absence of Vsx1 function, because the pan-bipolar markers Chx10 and Ret-B1 continued to be expressed in inner nuclear layer neurons labeled by the Vsx1-targeting reporter gene, τLacZ. The specification, number, and gross morphology of the subset of on-center and off-center (OFF)-CB cells defined by τLacZ expression from the Vsx1 locus were also normal in Vsx1τ LacZ/Vsx1τ LacZ mice. However, the terminal differentiation of OFF-CB cells in the retina of Vsx1τ LacZ/Vsx1τ LacZ mice was incomplete, as demonstrated by a substantial reduction in the expression of at least four markers (recoverin, NK3R, Neto1, and CaB5) for these interneurons. These molecular abnormalities were associated with defects in retinal function and documented by electroretinography and in vitro ganglion cell recordings specific to cone visual signaling. In particular, there was a general reduction in the light-mediated activity of OFF, but not on-center, ganglion cells. Thus, Vsx1 is required for the late differentiation and function of OFF-CB cells and is associated with a heritable OFF visual pathway-specific retinal defect.
Proceedings of the National Academy of Sciences of the United States of America © 2004 National Academy of Sciences