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Spatial Regulation of the cAMP-Dependent Protein Kinase during Chemotactic Cell Migration
Alan K. Howe, Linda C. Baldor, Brian P. Hogan and Susan S. Taylor
Proceedings of the National Academy of Sciences of the United States of America
Vol. 102, No. 40 (Oct. 4, 2005), pp. 14320-14325
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/3376737
Page Count: 6
You can always find the topics here!Topics: Physiological regulation, Phosphorylation, Endothelial cells, Antibodies, Animal migration behavior, Phosphatases, Good agricultural practices, Cultured cells, Chemotaxis, Cellular immunity
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Historically, the cAMP-dependent protein kinase (PKA) has a paradoxical role in cell motility, having been shown to both facilitate and inhibit actin cytoskeletal dynamics and cell migration. In an effort to understand this dichotomy, we show here that PKA is regulated in subcellular space during cell migration. Immunofluorescence microscopy and biochemical enrichment of pseudopodia showed that type II regulatory subunits of PKA and PKA activity are enriched in protrusive cellular structures formed during chemotaxis. This enrichment correlates with increased phosphorylation of key cytoskeletal substrates for PKA, including the vasodilator-stimulated phosphoprotein (VASP) and the protein tyrosine phosphatase containing a PEST motif. Importantly, inhibition of PKA activity or its ability to interact with A kinase anchoring proteins inhibited the activity of the Rac GTPase within pseudopodia. This effect correlated with both decreased guanine nucleotide exchange factor activity and increased GTPase activating protein activity. Finally, inhibition of PKA anchoring, like inhibition of total PKA activity, inhibited pseudopod formation and chemotactic cell migration. These data demonstrate that spatial regulation of PKA via anchoring is an important facet of normal chemotactic cell movement.
Proceedings of the National Academy of Sciences of the United States of America © 2005 National Academy of Sciences